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Microtome. A microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a cutting tool used to produce extremely thin slices of material known as sections, with the process being termed microsectioning. Important in science, microtomes are used in microscopy for the preparation of samples for observation under ...
Frozen section procedure. The frozen section procedure is a pathological laboratory procedure to perform rapid microscopic analysis of a specimen. It is used most often in oncological surgery. [1] The technical name for this procedure is cryosection. The microtome device that cold cuts thin blocks of frozen tissue is called a cryotome.
The cryostat is essentially an ultrafine "deli-slicer", called a microtome, placed in a freezer. The cryostat is usually a stationary upright freezer, with an external wheel for rotating the microtome. The temperature can be varied, depending on the tissue being cut usually from −20 °C to −30 °C. The freezer is either powered by ...
Ultramicrotomy. Ultramicrotomy is a method for cutting specimens into extremely thin slices, called ultra-thin sections, that can be studied and documented at different magnifications in a transmission electron microscope (TEM). It is used mostly for biological specimens, but sections of plastics and soft metals can also be prepared.
Similar to the frozen section procedure employed in medicine, cryosectioning is a method to rapidly freeze, cut, and mount sections of tissue for histology. The tissue is usually sectioned on a cryostat or freezing microtome. [12] The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues.
The Visible Human Project is an effort to create a detailed data set of cross-sectional photographs of the human body, in order to facilitate anatomy visualization applications. It is used as a tool for the progression of medical findings, in which these findings link anatomy to its audiences. [ 1] A male and a female cadaver were cut into thin ...
The laser microtome is an instrument used for non-contact sectioning of biological tissues or materials. It was developed by the Rowiak GmbH, a spin-off of the Laser Centre, Hannover . In contrast to mechanically working microtomes , the laser microtome does not require sample preparation techniques such as freezing, dehydration or embedding.
Uses. Flow cytometer. used for automated cell counting as in total blood count, differential count, etc. Tissue bath or organ bath or Dale's apparatus. used in full tissue experiments, for example using guinea pig ileum mainly used in pharmacology for application of drugs to these tissues. Sahli Haemoglobinometer.
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